The proteome of normal human chorionic villus sampling cells.
نویسندگان
چکیده
Chorionic villi samples are widely used for prenatal diagnosis of various fetal disorders. Although, our knowledge regarding the molecular level of these disorders is extensive, little is known about the implicated proteins. In the present study, two dimensional electrophoresis (2-DE) followed by mass spectrometry (MS) was applied to reveal the proteomic profile of the CV cells. This proteomic technique was previously used successfully in the cases of amniotic fluid, follicular fluid and maternal blood, but has not yet been applied to CV. Therefore, 2-DE was combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS) to characterise the proteome of normal CV cultured cells. Two hundred eighty two-individual gene products were identified including cytoplasmic and nuclear proteins. Although the majority of the proteins were enzymes, structural, signalling and carrier molecules were also isolated. 2D protein map elucidates 282 protein molecules expressed in the CV cells that can be used as a reference for future comparison to various pathological conditions.
منابع مشابه
Chorionic villus sampling for the diagnosis of beta thalassemia in the first trimester of pregnancy in southwest Iran
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Invasive prenatal diagnosis techniques include chorionic villus sampling (CVS), amniocentesis, cordocentesis or percutaneous umbilical blood sampling (PUBS), fetal tissue sampling, as well as embryoscopy and fetoscopy (Table 2). Some diagnostic results may be obtained by more than one technique: for example, fetal karyotype can be obtained from cells from amniocentesis, chorionic villus samplin...
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عنوان ژورنال:
- In vivo
دوره 25 6 شماره
صفحات -
تاریخ انتشار 2011